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KMID : 1189420110240010016
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2011 Volume.24 No. 1 p.16 ~ p.24
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Effect of Anemarrhenae Rhizoma Ethanol Extract on Apoptosis Induction of HT-29 Human Colon Cancer Cells
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Kim Tae-Hyun
Kim Pom-Ho
Jeon Byoung-Kook
Yoon Jeong-Rock
Woo Won-Hong
Mun Yeun-Ja
Lee Jang-Cheon
Lee Boo-Kyun
Park Young-Gyu
Lim Kyu-Sang
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Abstract
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Objective : In this study, we investigated the effects of ethanol extract of Anemarrhenae Rhizoma (EAR) on the proliferation and apoptosis induction of HT-29 human colon cancer cells.
Methods:Cell viability of HT-29 cells were measured by MTT assay and apoptisis-related proteins were assessed using western blotting. Chromatin condensation of HT-29 cells stained with Hoechst 33258.
Results: In the present study, we demonstrated that EAR exhibited significant cytotoxicity in HT-29 cells. The induction of apoptosis in HT-29 cells by EAR treatment was characterized by chromatin condensation and the activation of caspase-3. EAR-induced apoptosis is accompanied by the release of cytochrome c and the specific proteolytic cleavage of PARP. EAR was appeared cytotoxic effect to HT-29 cells in a dose-dependent manner. Concomitantly, EAR treatment led to increase in the caspase-9. The reduction of Bcl-2 and truncation of Bid were induced by EAR.
Conclusion:We studied that the EAR induced apoptosis in human colon adenocarcinoma HT-29 cells. These results indicated that EAR can cause apoptosis through mitochondria/caspase pathway in human HT-29 cells.
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KEYWORD
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Colon cancer, Apoptosis, Mitochondria, Caspase, Bcl-family
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